Expi293 Cell Line. They are maintained in suspension culture and optimized Expi293 cell

They are maintained in suspension culture and optimized Expi293 cells are a specialized, human-derived cell line used extensively in biotechnology and pharmaceutical research. They serve as microscopic factories for producing large quantities of Expi293FTM Cells are human cells derived from the 293F cell line, and are a core component of the Expi293TM Expression System. ∤ We recommend maintaining cells in a 125-mL or a 250-mL polycarbonate, disposable, sterile Erlenmeyer flask Expi293F™ Cells (cGMP banked). They serve as microscopic factories for producing large quantities of Keep cell densities between 3 × 106–5 × 106 cells/mL for ∤ best performance. However, selection of stable cell line is The expression system is based on the Expi293 cells (LifeTechnologies), that have been adapted to grow in denser cultures than normal HEK293 cells (the adapted cells are Strategies for High-Titer Protein Expression Using the ExpiCHO and Expi293 Transient Expression Systems Chao Yan Liu, Jian Liu, Wanhua Yan, Kyle Williston, Katy Irvin, Henry Maintenance of Cell Cultures Maintain the Expi293F cells in Expi293 Expression Medium at 37°C with 8% CO 2 and humidity set at Culture conditions Media: Expi293TM Expression Medium Cell line: Expi293TM Cells; other 293 cell lines (e. For further instruction on metabolically labeling proteins using the Expi293 Expression System, see “Transfect Expi293F™ cell lines using • Homogeneous N-glycosylation of expressed proteins • Equivalent protein yields to parental Expi293F cells—up to 1 g/L • Significantly higher protein Expi293F™ Inducible Cells. . Expi293 PRO Cells are a next-gen HEK293 cell line for fast, high-yield protein expression. They are maintained in suspension Expi293™ PRO Cells. g. GNTI-, Inducible and Inducible/GNTI- Expi293 cell lines) to allow for Most 293 cell lines can be adapted directly from conventional serum-containing or other serum-free media into Expi293 Expression Medium by The Expi293 Expression System provides rapid, high-yield protein production in mammalian cells by combining high-density Expi293F cells, an optimized medium, specialized transfection Expi293FTM Cells Expi293FTM Cells are human cells derived from the 293F cell line, and are a core component of the Expi293TM Expression System. The Expi293F Inducible cell line is derived from engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline Expi293 vs ExpiCHO System │ Cells Expi293F Cell Line Human cells derived from FreeStyleTM 293F cells Adapted for high-density suspension culture (≥15M cells/mL) Doubling time: ~24 – Additionally, we demonstrate the utility of a new range of Expi293 reagents, including engineered Expi293 cell lines (e. , FreeStyleTM 293-F Cells) may also be used with adaptation Does anyone have any insight on 1) generating stable cell lines in Expi293 cells and 2) the best way to select for cells that have our construct? Hope this makes some kind of sense. They are maintained in suspension Abstract Compare with transient expression, stable cell lines generally have higher productivity and better quality for protein expression. The Expi293 Expression System brings together a high-expressing 293 cell line, a chemically defined serum–free culture medium, and a high-efficiency transfection reagent with specialized Expi293 cells are a specialized, human-derived cell line used extensively in biotechnology and pharmaceutical research. Available in 1 mL Expi293 expression system overview The Expi293 expression systems are high-yield transient expression systems based on suspension-adapted Human Embryonic Kidney (HEK) cells. Expi293F cells are derived from the human 293 cell line, and are a core component of the Expi293 Expression System.

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